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Aptamer 25 displaces bound primer-template from polβ. Gel shift competition reactions were carried out as described in Materials and Methods. Each reaction contained 250 nM 4-base gapped DNA, 700 nM polβ (except lane 2) and the indicated amount of purified aptamer 25; lanes 1–3, no aptamer; lanes 4–9, 6.0 µM, 3.0 µM, 1.5 µM, 0.75 µM, 0.38 µM and 0.19 µM. The positions of free and bound labeled primer-templates are indicated on the right.
