Table 1.
RT-PCR analyses using primers against differentially expressed sequences and multiple tissue cDNA panels
| Primers against clone, homologous to | se2-1 (SCP-1) | se14-2 (cTAGE-1) | se20-10 (⊘) | se33-1 (NP220) | se57-1 (⊘) | se70-2 (⊘) |
|---|---|---|---|---|---|---|
| Bone marrow | − | − | + | + | + | − |
| Brain | − | − | + | + | − | + |
| Colon | − | − | − | − | + | − |
| Fetal brain | − | − | − | + | − | + |
| Fetal heart | − | − | − | + | − | + |
| Fetal kidney | − | − | + | + | − | + |
| Fetal liver | − | − | − | − | − | + |
| Fetal lung | − | − | − | + | − | + |
| Fetal skeletal muscle | − | − | − | + | − | + |
| Fetal spleen | − | − | − | + | − | + |
| Fetal thymus | − | − | − | − | − | + |
| Heart | − | − | + | − | − | − |
| Kidney | − | − | + | + | − | + |
| Liver | − | − | − | + | − | − |
| Lung | − | − | − | + | − | − |
| Ovary | − | − | − | + | − | − |
| Pancreas | − | − | − | + | − | − |
| Peripheral blood lymphocytes | − | − | − | − | − | − |
| Placenta | − | − | + | + | − | − |
| Prostate | − | − | + | + | − | − |
| Skeletal muscle | − | − | − | − | − | − |
| Skin | − | − | − | + | − | − |
| Small intestine | − | − | + | + | + | + |
| Spleen | − | − | + | + | + | + |
| Stomach | − | − | + | + | − | − |
| Testis | + | + | + | + | + | + |
| Thymus | − | − | − | + | − | + |
| Trachea | − | − | + | + | + | + |
Either commercially available cDNA panels or cDNA generated from RNA panels (both from CLONTECH) were used for RT-PCR. Each probe consists of several donor tissues of the indicated type. cDNA from skin tissue was generated by RNA isolation of one specimen. For details see Material and Methods. ⊘, No homologous sequences found.