Table 4.
RT-PCR analyses using antigen-specific primers and cDNA from various tissues and cell lines
| cDNA, primers against clone | Controls
|
Tumor tissues (CTCL, n) | Cell lines
|
|||
|---|---|---|---|---|---|---|
| Multi-tissue panels* (n) | Activated CTLs† (n = 3) | CTCL (n = 4) | Leukemia (n = 6) | Melanoma (n) | ||
| se1-1 | 100% (16) | 100% | 91% (11) | 100% | 100% | 100% (6) |
| se2-1 | 4% (28)‡ | 0% | 6% (17) | 0% | 0% | 0% (11) |
| se2-2 | 100% (16) | 100% | 90% (10) | 100% | 83% | 80% (5) |
| se2-5 | 100% (16) | 0% | 55% (11) | 0% | 100% | 100% (11) |
| se14-2 (cTAGE-1) | 4% (28)‡ | 0% | 35% (17) | 100% | 0% | 0% (20) |
| se14-3 | 100% (16) | 100% | 100% (11) | 100% | 100% | 100% (11) |
| se20-4 | 100% (16) | 100% | 92% (12) | 100% | 100% | 100% (11) |
| se20-7 | 100% (16) | 100% | 100% (11) | 100% | 50% | 80% (5) |
| se20-9 | 100% (16) | 100% | 82% (11) | 100% | 83% | 45% (11) |
| se20-10 | 43% (28)‡ | 67% | 77% (13) | 100% | 100% | 55% (11) |
| se33-1 | 79% (28)‡ | 100% | 75% (16) | 100% | 83% | 100% (11) |
| se37-2 | 100% (16) | 100% | 93% (15) | 100% | 83% | 75% (5) |
| se57-1 | 21% (28)‡ | 0% | 6% (17) | 0% | 0% | 0% (23) |
| se70-2 | 54% (28)‡ | 33% | 31% (16) | 100% | 100% | 45% (22) |
| se89-1 | 79% (14) | 100% | 75% (16) | 100% | 100% | 73% (22) |
Table gives numbers as percentage of positive tissues or cell lines when analyzed by RT-PCR using clone-specific primers and the total number of tested specimen (n) in parentheses. Differentially expressed and cancer-germline clones are bold.
*
RT-PCRs using testis cDNA gave always positive results. In the case of clones se2-1 and se14-2 cDNA from testis tissue was the only positive probe. Content of panels see Table 1.
†
Activated cytotoxic T-cells (see material and methods).
‡
For specific results on these clones see Table 1.