Fig. 3.
Distinct and complementary contributions of mda-5 and TLR3-TRIF to polyI:C-induced responses in thioglycollate-induced peritoneal macrophages. Macrophages (2 × 105 per well) from WT and TRIFLPS2 mice (a) or WT and mda-5−/− mice (b) were collected 48 h after i.p. thioglycollate injection and stimulated with polyI:C either naked (100 μg/ml) or in complex with Fugene (1.5 μg/ml). After 24 h, cell culture supernatants were assessed for IFN-α by ELISA and for TNF-α, MCP-1, and IL-6 by cytokine bead array. Data shown are representative of two independent experiments.