Fig. 5.

mda-5 is critically required for type I IFN and cytokine response to EMCV. Bone marrow-derived DC (a, b, and d), bone marrow-derived macrophages (a, b, and d), and thioglycollate-induced peritoneal macrophages (c and d) from WT (filled symbols) and mda-5-deficient (open symbols) mice were stimulated in vitro with EMCV or MCMV at indicated multiplicities of infection. Production of IFN-α (a and c) and IL-6 and MCP-1 (b and c) in response to EMCV. (d) IFN-α response to MCMV. Cell culture supernatants were examined for IFN-α by ELISA and for IL-6 and MCP-1 by cytokine bead array. Data shown are representative of two independent experiments.