Figure 1.

Dendrograms showing gene expression measured by qRT-PCR on RNA from 18 Sézary patients with high blood tumor burden and 12 healthy controls, including 3 untreated and 9 skewed to Th2 phenotype. Hierarchical clustering was applied to both the genes and the samples and was performed using Pearson correlation–based distance metric and Ward linkage. Samples from SS patients, untreated controls, and Th2-skewed controls are colored in red, green, and blue, respectively. Genes up- and down-regulated in the patients as compared with Th2-skewed controls are colored red and green, respectively. For visual enhancement, expression levels for each gene are converted to z scores. (A) Dendrogram of qRT-PCR on 26 of the genes with P < .01 measured by qRT-PCR on amplified RNA. (B) Dendrogram of 5 genes selected for our discriminant model on the same set of 30 samples assayed by qRT-PCR on total RNA derived from the same samples.