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. 2006 May 19;34(9):e71. doi: 10.1093/nar/gkl358

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© The Author 2006. Published by Oxford University Press. All rights reserved

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Detection and characterization of the mazF gene, the mpd expression cassette, the partial amyE gene and the bpr gene using PCR. Plasmids or B.subtilis chromosomal DNA were used as the template, and the amplified fragments were analyzed by agarose gel electrophoresis. Lanes 1 and 13, DNA markers. (A) The primer pair P5/P6 was used to amplify the mazF gene. Lane 2, pDGIEF (positive control); lane 3, 1A751 (negative control); lane 4, BS752; lane 5, BS752S. (B) The primer pair P11/P12 was used to amplify the mpd expression cassette. Lane 6, pDGIEF-mpd (positive control); lane 7, BS752S (negative control); lane 8, BS753S. (C) The primer pair P22/P23 was used to amplify the partial amyE gene. Lane 9, BS752S (negative control); lane 10, BS753S. (D) The primer pair P13/P16 was used to amplify the bpr gene. Lane 11, BS753S (negative control); lane 12, BS754S.