Table 1.

Strains and plasmids

Strain or plasmid	Characteristicsa	Reference
E.coli JM109	recA1, supE44 endA1 hsdR17 (${\text{r}}_{\text{k}}^{-}$, ${\text{m}}_{\text{k}}^{+}$) gyrA96 relA1 thi (lac-proAB) F′[traD36 proAB+lacIqlacZΔM15]	(9)
B.subtilis
1A751	his nprR2 nprE18 ΔaprA3 ΔeglS102 ΔbglT bglSRV	(10)
BS752	1A751 derivate, Spcr, amyE::(DR, lacI, mazF, spc, DR)	This work
BS752S	1A751 derivate, amyE::DR	This work
BS753	1A751 derivate, Spcr, amyE::(mpd, DR, lacI, mazF, spc, DR)	This work
BS753S	1A751 derivate, amyE::(mpd, DR)	This work
BS754	1A751 derivate, Spcr, amyE::(mpd, DR), bpr::(DR, lacI, mazF, spc)	This work
BS754S	1A751 derivate, amyE::(mpd, DR), Δbpr	This work
Brevibacillus brevis B15	Wild-type	Stored in this lab
Plasmids
pDG148	Ampr, Kmr, B.subtilis–E.coli shuttle expression vector	(11)
pDG1730	Ampr, Spcr, Ermr, B.subtilis amyE integration vector	(12)
pDGIEF	Ampr, Spcr, B.subtilis amyE integration vector	This work
pP43NMK	Ampr, Kmr, B.subtilis–E.coli shuttle expression vector containing an expression cassette including ytkA promoter, NprB signal peptide-encoding sequence and mpd gene	(13)
pP15MK	Ampr, Kmr, B.subtilis–E.coli shuttle expression vector	This work
pDGIEF-mpd	pDGIEF with mpd expression cassette inserted in NotI/NheI site	This work
pBluescript II SK(−)	Ampr, E.coli cloning vector	Stratagene
pIEFBPR	Ampr, Spcr, B.subtilis bpr integration vector	This work
pIEFBPR-ID	Ampr, Spcr, B.subtilis bpr integration vector	This work

^aSpc^r: Spectinomycin-resistance, Amp^r: Ampicillin-resistance, Km^r: Kanamycin resistance, Erm^r: Erythromycin resistance.