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. 2006 May 3;7:104. doi: 10.1186/1471-2164-7-104

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Copyright © 2006 Hurley et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Southern hybridisation analysis of VPaI-1 negative strains 428/00 and 30824. PaI-1-negative strains KE9984 and KE9967, VPaI-1-positive strain RIMD2210633 and VPaI-1-negative strains 30824 and 428/00. Genomic DNA was restricted using EcoRI and the probe used was constructed from the PCR amplification using primer pair 379F and 404R with strain KE9984 as template. The expected 8 Kb and 11.4 kb band sizes in the VPaI-1 negative strains KE9984 and KE9967 are shown (Lane 1–2). The VPaI-1 positive RIMD2210633 gave the expected 11.4 Kb, 6.6 Kb and 3.1 Kb band sizes (Lane 3). The VPaI-1 negative strains 30824 and 428/00 with novel DNA inserted gave the expected band sizes of 13 Kb and 11.4 Kb.