Figure 1. Effect of fasting and feeding upon the expression and molecular functional status of AMPK and ACC in the hypothalamus.

The hypothalami of anaesthetized rats maintained on an unrestricted offer of diet (Fed), or food restricted for 12 h (Fast) were homogenized on ice-cold extraction buffer. Aliquots of protein extracts containing 0.2 mg protein were separated by SDS-PAGE, transferred to nitrocellulose membranes and blotted (IB) with anti-phospho-[Thr¹⁷²] AMPK (pAMPK) (A, upper blot) or anti-AMPK (A, lower blot), or anti-phospho-[Ser⁷⁹] ACC (pACC) (B, upper blot) or anti-ACC (B, lower blot). Specific bands were quantified by scanning densitometry and means ± s.e.m. are plotted as arbitrary scanning units. In all experiments, n = 5, *P < 0.05 versus Fed.