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. 2005 Sep 8;568(Pt 3):891–903. doi: 10.1113/jphysiol.2005.093047

Figure 3. TEPIQ analysis of ΔV.

Figure 3

A and B, fluorescence images (xz and xy, respectively) of a single β-cell on a cover slip immersed in a solution containing SRB (0.4 mm). C, the volume of an exocytic vesicle is estimated from the SRB fluorescence intensities for an exocytic event (FV) and for a unit volume of extracellular solution (F′E). D, an actual point spread function for a quantum dot (diameter of 5 nm) along the z-axis (•). The fluorescence intensity was normalized so that the total integral became 1.0. The smooth line is predicted by eqn (A17) in Appendix C.