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. 2001 Jan 16;98(2):646–651. doi: 10.1073/pnas.98.2.646

Figure 5.

Figure 5

Clonal analysis of growth arrest. (A) Relative CE of the four fibroblast cultures at 33.5°C after 0 days (open bars), 3 days (▨), 7 days (cross-hatched bars), 10 days (▧), and 14 days (solid bars) at 39.5°C. Results are means of duplicate determinations (variance of replicate CE < ± 10%). (B) Relative colony-forming efficiency at 33.5°C of the endothelial culture HMME7 after 0 days (open bars), 3 days (▨), 6 days (cross-hatched bars), and 9 days (▧) at 36.5°C and at 39.5°C, respectively. Results are means ± SD (n = 4). The corresponding numbers of SA β-galactosidase positive cells were 5% (day 0), 11% (day 3), 54% (day 6), and 79% (day 9) in cultures at 36.5°C, compared with 3% (day 3), 6% (day 6), and 12% (day 9) in cultures at 39.5°C.