Figure 7. Effect of stimulation on frequenin expression.

A, Western blot showing the expression of frequenin in tonic sartorius and phasic CP frog muscles and in frog brain tissue. Frequenin protein was poorly expressed in sartorius muscle. B, Western blot showing the expression of frequenin in stimulated and control CP muscles of 3 frogs and in frog brain tissue. Frog 2 and 3 presented a decrease in frequenin expression in stimulated muscles whereas frog 1 showed no obvious difference between control and stimulated muscles. C, characteristic frequenin staining in control and stimulated NMJs. NMJs were visualized using Bodipy-conjugated α-bungarotoxin to reveal the distribution of nAChRs. The merged image in side view reveals that anti-frequenin staining is found directly above the nAChRs, in a pattern consistent with staining of the nerve terminal.