Skip to main content
. 2005 Oct 13;569(Pt 2):447–457. doi: 10.1113/jphysiol.2005.095083

Figure 2. N6-modified ATP analogues are more potent than ATP.

Figure 2

A, after patch excision, wild-type cystic fibrosis transmembrane conductance regulator (WT-CFTR) channels were activated by protein kinase A (PKA) and 1 mm ATP. ATP (2.75 mm) was then applied before and after the application of each ATP analogue (5 μm). B, mean current responses to 2.75 mm ATP and 5 μm ATP analogues of WT-CFTR channels were compared. The ratios of the mean current induced by ATP analogue (5 μm) to that by 2.75 mm ATP for WT-CFTR channels are 1.12 ± 0.09 (n = 10), 0.54 ± 0.05 (n = 10) and 0.25 ± 0.04 (n = 8) for P-ATP, B-ATP and M-ATP, respectively. This ratio is 0.07 ± 0.04 (n = 8) for 5 μm ATP (data from Zeltwanger et al. 1999). For ΔR-CFTR channels, this ratio is 1.09 ± 0.06 (n = 9) for 5 μm P-ATP.