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. 2005 Oct 20;569(Pt 3):751–760. doi: 10.1113/jphysiol.2005.097477

Figure 4. Blockade of cannabinoid reuptake increased endocannabinoid levels.

Figure 4

A, continuous recording of mEPSCs (1) and running average plot of mEPSC frequency (2) from the same SON magnocellular neurone showing that the cannabinoid transporter blocker OMDM-2 (5 μm) caused a decrease in mEPSC frequency. B, continuous recording of mEPSCs (1) and running average plot of mEPSC frequency (2) from another SON neurone showing blockade of the OMDM-2-induced suppression of mEPSCs by the CB1 antagonist AM251 (1 μm). C, average changes in the mean mEPSC frequency caused by OMDM-2 (n = 5) and OMDM-2 in AM251 (n = 5). D, HFS of trimmed SON slices in the presence of the cannabinoid transporter blocker AM404 (1 μm) elicited a robust increase in the levels of both AEA and 2-AG compared to controls, measured by LC-MS-MS (n = 5). *P < 0.05; **P < 0.01.