Figure 7. Expression of glycine and GABA receptor subunits.

A, RT-PCR detected GABA receptor α₁, α₂, α₃, α₄ and α₅ and glycine receptor α₂ and α₃ expression in the GLUTag cell line, but not GABA receptor α₆ and glycine receptor α₁. Expression of all α subunits was detectable when whole mouse brain RNA was used as a template (right-hand side) and no bands were detectable when H₂O was used as a template (not shown). Predicted band sizes are as indicated. The marker in the centre lane of the gel has a band every 100 bp from 200 to 1000 bp. B, nested RT-PCR detected GABA receptor ρ₁, ρ₂ and ρ₃ subunit expression in the GLUTag cell line, and ρ₁ and ρ₂ subunit in mouse brain. Predicted band sizes are as indicated. The marker in the centre lane of the gel has a band every 100 bp from 200 to 1000 bp.