Figure 7. Comparison of ‘delayed replacement’ (40 s delay) and ‘rapid replacement’ (1.2 s delay) paired filtration study protocols.

A, rapid replacement resulted in no significant change of initial glomerular volume (P > 0.8, one-sample t test versus 0), in contrast to delayed replacement (filled column). B, rapid replacement, in contrast to delayed replacement, resulted in no ejection of erythrocytes (P > 0.35, one-sample t test versus 0). C, there was no significant change in L_pA per unit volume (L_pA/V_i, min⁻¹ mmHg⁻¹) between the first and second filtrations using both the ‘delayed replacement’ and ‘rapid replacement’ protocols. D, there was a significant change in the mean rate of transglomerular fluid efflux between the first (dark filled column) and second (light filled column) repetition of filtration in populations of glomeruli subjected to both the ‘delayed replacement’ (*P < 0.05, unpaired t test, n = 5) and ‘rapid replacement’ (**P < 0.005, unpaired t test, n = 5) protocols.