Table 2.

Summary of the major differences between the work of Savin et al. and the current study

	Savin et al.*	Current study
Apparatus	Room temperature, open bath	37°C, closed system
Application of oncotic gradient	Gravity-fed replacement of static solution at uncontrolled rate	Controlled, continuous flow; rapid/reproducible switch
Direction of transglomerular fluid flux	Into glomerulus – non-physiological	Leaving glomerulus – physiological
Repetitive filtration studies	Crimped arterioles (rarely published)	Double perifusate switch
Estimation of glomerular volume	Four equally spaced glomerular diameters	Glomerular perimeter
Estimation of initial rate of change of glomerular volume	Greatest difference in glomerular volume occurring in 0.017 s	Actual rate of change in glomerular volume occurring in 0.04–0.1 s
Units of measurement	LpA	LpA/Vi, mean Jv(f)

^*Savin & Terreros (1981); Pinnick & Savin (1986).