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. 2006 Apr 28;103(19):7252–7257. doi: 10.1073/pnas.0600862103

Fig. 4.

Fig. 4.

Analysis of interaction between StrepIIVirB8sp variants and StrepIIVirB10sp by crosslinking. (A) StrepIIVirB8sp and variants were mixed with StrepIIVirB10sp and incubated with 0.1 mM DSS followed by SDS/PAGE and Western blotting with VirB8s-specific antiserum. Crosslinked products of StrepIIVirB8sp in the absence of StrepIIVirB10sp are indicated by ∗, the occurrence of a novel product in the presence of StrepIIVirB10sp is indicated by an arrow, and arrowheads indicate reduced amounts of this presumptive heterodimer in case of two variants as compared with WT (representative results of three repetitions are shown). (B) Western blots of SDS gels from crosslinked samples as loading controls. An additional VirB8sp-specific signal of lower molecular mass (indicated by a triangle) was observed in the presence of DSS that may correspond to an internal crosslink causing the change of migration. Molecular masses of reference proteins are shown on the right (in kDa).