Fig. 4.
Stereoview of active sites. (A) Overlay of the active sites of Cu(II) AZAMI (green), AZAMI-F (gray), AZ (purple), and AMI (yellow). The side chains of the coordinating residues and the amino acids on either side of the N-terminal His ligand are shown as stick models, copper atoms as spheres, and the backbone of the C-terminal ligand-containing loops as Cα traces. The residues are labeled as in AZ. (B) Overlay of the active sites of the Cu(I) form of AZAMI-F at pH 8 (slate) and pH 6 (cyan) is shown. The second conformation of the copper ion and His-115 at pH 6 are colored green. The figure was prepared by using the program pymol (6).