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. 2006 May 1;103(19):7333–7338. doi: 10.1073/pnas.0508419103

Fig. 6.

Fig. 6.

Effect of anti-DHP antibody treatment on 11-KT-induced spermatogenesis in a germ cell/somatic cell coculture system. (A and B) Microphotographs show the pellets of germ cells/somatic cells cultured in basal medium alone (A) and 11-KT and anti-DHP antibody together (B) in long-term culture. Dark cells are BrdU-positive cells. (Scale bar, 50 μm.) (C) BrdU index. The number of positively immunoreacted germ cells is expressed as a percentage of the total number of germ cells. IC, initial control; C, negative control without hormone; KT, 10 ng/ml 11-KT; DHP, 1 ng/ml DHP; +, with anti-DHP antibody; −, without anti-DHP antibody. Results are given as means ± SEM calculated for each of three samples. Values with different letters are significantly different (P < 0.05). There are no letters on the graph.