Skip to main content
. 2006 May 1;103(19):7333–7338. doi: 10.1073/pnas.0508419103

Fig. 7.

Fig. 7.

Effect of DHP on induction of meiosis in the Japanese eel in vitro. (A) Expression of meiosis-specific marker proteins Spo11 and Dmc1 in cultured testicular fragments. Testicular fragments were cultured with 0.1, 1, 10, or 100 ng/ml DHP or 1, 10, or 100 ng/ml 11-KT. An initial control sample taken before culture and a control cultured without hormone were run in lanes IC and C, respectively. As a positive control for the expression of meiosis-specific markers, testis tissue fragments cultured with 10 ng/ml KT for 20 days and containing numerous spermatocytes was run in lane 20D-KT. Western blots were repeated five times, using tissue from five males; similar results were obtained in all cases. (B–D) Microphotographs show anti-eelSpo11 immunoreactive material in testicular fragments cultured without hormone (B), with 10 ng/ml 11-KT (C), or with 10 ng/ml DHP (D) for 6 days. (Scale bar, 10 μm.) (E) Electron micrograph of germ cells with synaptonemal complexes (arrows) in testicular fragment cultured with 10 ng/ml DHP for 6 days. (Scale bar, 10 μm.) (F) Percentages of Spo11-positive germ cells. The number of immunoreactive germ cells is expressed as a percentage of the total number of germ cells. IC, initial control; C, control without hormone. Results are given as means ± SEM calculated for each of five samples.