Fig. 1.

MG132 alters rhTRIM5α cytoplasmic localization. (A) rhTRIM5α.HA forms cytoplasmic bodies in stable HeLa cells (Left) that accumulate into larger bodies after 5 h of MG132 treatment (Right). (B) Western blot analysis reveals huTRIM5α.HA (Left) or rhTRIM5α.HA (Right) expression remains similar in HeLa cells after 6 h of MG132 treatment, despite declining with the translation inhibitor cyclohexamide (CHX). (C) Similar rhTRIM5α.HA stability without (Upper Inset; ♦) or with (Lower Inset; ▵) MG132 by pulse–chase analysis. Immunoprecipitated rhTRIM5α.HA bands are shown over time, with their percent intensity relative to the 0-min chase graphed.