Fig. 4.
MG132 allows extensive RT of HIV-1 cDNA despite exogenous rhTRIM5α. (A) Location of DNA digest sites and probes for Southern blot analysis of HIV-1 5′ (probe 1) and 3′ (probe 2) cDNA ends. (B–D) Southern blot analysis of uninfected (−) or R7-GFPHIV-1 infected HeLa or rhTRIM5α.HA stable HeLa (Rh HA) cells without (V) or with MG132 (MG). The same blot probed for 5′ (B) or 3′ (C) HIV-1 cDNA or GAPDH for DNA loading (D) is shown. Asterisks mark residual probe 2. Although late RT products with complete ends escape rhTRIM5α restriction via MG132, 1-LTR and 2-LTR circles remain impaired.