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. 2006 Apr 28;103(19):7471–7475. doi: 10.1073/pnas.0602057103

Fig. 2.

Fig. 2.

Progressive loss of spinal cord motor neurons in Zpr1+/− mice. (A) Reduced Zpr1 gene dosage causes decreased expression of ZPR1 protein in the spinal cord. The expression of ZPR1 in the spinal cords of 6-week-old and 12-month-old WT and Zpr1+/− mice was examined by immunoblot analysis and quantitated by using metamorph software (mean ± SD; four mice per group). (B) Staining with hematoxylin and eosin (Upper) and an antibody to SMN (Lower) of the anterior horns of the lumbar region of spinal cords from 12-month-old WT and Zpr1+/− mice (scale bar, 100 μm). Void spaces are indicated (arrows). (C) ZPR1 deficiency causes loss of spinal cord motor neurons. Quantitation of the number of spinal motor neurons in serial sections of spinal cords from 6-week-old and 12-month-old WT and Zpr1+/− littermates was performed. Motor neurons were counted in every fifth section of the lumbar (L1–L5) region of the spinal cords (mean ± SD; six mice per group). The loss of motor neurons was calculated by using number of motor neurons in 6-week-old mice (100%) as reference point. (D) Progressive loss of spinal cord motor neurons in Zpr1+/− mice. Quantitation of motor neuron numbers in serial sections of the spinal cords from WT and Zpr1+/− littermates. Motor neurons were counted in every fifth section of the thoracic (T9–T12) region of spinal cords (mean ± SD; six mice per group). The loss of motor neurons was calculated by using number of motor neurons in 6-week-old mice as the reference point (100%).