Table 2.
Leaf lipid composition of Arabidopsis WT and dgd1 mutant plants expressing βGlcT from Chloroflexus
| Lipid | WT | WT-βGlcT | dgd1 | dgd1-βGlcT | dgd1-DGD1 |
|---|---|---|---|---|---|
| βGalDG | 52.5 ± 2.2 | 46.6 ± 4.0 | 42.3 ± 1.4 | 45.3 ± 1.1 | 50.7 ± 5.8 |
| PG | 6.8 ± 0.6 | 7.6 ± 0.6 | 13.7 ± 0.9 | 9.8 ± 0.6 | 7.8 ± 1.7 |
| βGlcβGalDG | —* | 8.7 ± 0.2 | —* | 17.7 ± 1.2 | —* |
| αGalβGalDG | 16.4 ± 0.3 | 18.2 ± 1.7 | 1.5 ± 0.3 | 2.7 ± 0.7 | 15.3 ± 1.1 |
| SQDG | 2.9 ± 0.5 | 4.6 ± 0.8 | 3.6 ± 0.6 | 6.5 ± 1.8 | 2.9 ± 2.0 |
| PE | 7.6 ± 0.9 | 6.3 ± 1.9 | 14.6 ± 1.6 | 5.4 ± 2.0 | 8.1 ± 1.1 |
| PC | 13.8 ± 1.5 | 8.1 ± 2.9 | 24.4 ± 1.5 | 12.6 ± 0.8 | 16.1 ± 0.8 |
Lipids were separated by TLC, and fatty acids were quantified by GC of methyl esters. Data (in mol%) represent means ± SD of three measurements. Data for dgd1-DGD1 (line R376) were taken from ref. 27. PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; SQDG, sulfoquinovosyldiacylglycerol.
*Below detection limit.