Table 3.
Fatty acid | βGalDG |
βGlcβGalDG |
αGalβGalDG |
|||||||
---|---|---|---|---|---|---|---|---|---|---|
WT | WT-βGlcT | dgd1 | dgd1-βGlcT | WT-βGlcT | dgd1-βGlcT | WT | WT-βGlcT | dgd1 | dgd1-βGlcT | |
16:0 | 1.1 | 0.7 | 5.1 | 3.6 | 2.0 | 3.7 | 12.1 | 5.6 | 25.5 | 24.4 |
16:1 | 0.9 | 0.1 | 3.7 | 1.0 | 0.7 | 0.5 | 0.3 | 0.2 | 4.5 | 0.8 |
16:2 | 0.1 | 0.5 | 0.6 | 0.1 | 0.6 | 0.1 | 0.6 | 0.2 | 1.7 | 0.1 |
16:3 | 35.1 | 31.7 | 15.6 | 22.2 | 14.6 | 19.0 | 3.4 | 2.1 | 3.5 | 5.7 |
18:0 | 0.3 | 0.1 | 0.6 | 0.3 | 0.5 | 0.4 | 0.8 | 0.4 | 3.8 | 3.7 |
18:1 | 1.0 | 0.1 | 1.0 | 0.8 | 0.2 | 0.4 | 0.6 | 0.1 | 5.0 | 1.6 |
18:2 | 1.9 | 1.3 | 2.6 | 1.9 | 2.4 | 1.6 | 4.1 | 2.8 | 9.0 | 12.5 |
18:3 | 59.5 | 65.6 | 70.1 | 70.1 | 78.9 | 74.3 | 77.9 | 88.5 | 47.2 | 51.0 |
Lipids were isolated by TLC, and fatty acid composition was determined by GC of methyl esters. Data are given in mol% and represent means of three experiments. SD was always <2.0 mol%. Fatty acids are abbreviated as X:Y, where X and Y depict the number of carbon atoms and double bonds, respectively.