Table 4.
Prokaryotic and eukaryotic molecular species of leaf glycolipids
| Fatty acid composition | βGalDG |
βGlcβGalDG |
αGalβGalDG |
|||
|---|---|---|---|---|---|---|
| WT | WT-βGlcT | WT | WT-βGlcT | WT | WT-βGlcT | |
| C16 at sn-2 | 73.0 ± 1.3 | 68.1 ± 2.4 | — | 31.9 ± 2.0 | 11.8 ± 0.5 | 13.2 ± 1.5 |
| C18 at sn-2 | 26.7 ± 1.6 | 31.8 ± 2.4 | — | 67.6 ± 1.7 | 88.1 ± 0.5 | 86.6 ± 2.5 |
Glycolipids isolated by TLC were digested with R. arrhizus lipase, and reaction products were separated by TLC. The fatty acids of lysoglycolipids were measured by GC after transmethylation and indicate the fatty acid composition (total C16/prokaryotic species or total C18/eukaryotic species) at the sn-2 position. Data (in mol%) represent means ± SD of three experiments.