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. 2006 May 26;2(5):e48. doi: 10.1371/journal.ppat.0020048

Figure 1. Changes in the Transcription of Other Amoebapore-Like Genes in G3 Trophozoites as Determined by RT-PCR.

Figure 1

(A) Total RNA was prepared from freshly harvested HM1-IMSS and G3 trophozoites. The RNA was treated with RNase-free DNase, and reversed transcribed using oligo dT-adaptor primer (Table 1, primer I). PCR was then performed using a sense primer from the gene of interest (Table 1) and the adaptor primer (Table 1, primer II) as antisense for all the genes. Lanes: 1, EhRPL21; 2, Ehap-a; 3, Ehap-b; 4, Ehap-c; 5, SAPLIP 1; 6, SAPLIP 5; 7, SAPLIP 14.

(B) Sequence comparisons between the three genes that were silenced: I, Ehap-a; II, Ehap-b; III, SAPLIP 1.