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. Author manuscript; available in PMC: 2006 May 22.
Published in final edited form as: Blood. 2005 Jan 6;105(8):3222–3229. doi: 10.1182/blood-2004-11-4205

Figure 4.

Figure 4

Involvement of adenosine, LTB4, and chemokines in an autocrine/paracrine mechanism of IgEand IgE+Ag-induced mast cell migration. (A) CFSE-labeled wild-type and Fc∈;RIα−/− BMMCs in upper wells were attracted to lower wells containing wild-type cells and SPE-7 or 206 IgE–sensitized wild-type cells and antigen. (B) Supernatants of wild-type BMMCs incubated with SPE-7 for the indicated periods attracted Fc∈;RIα−/− cells. Supernatants of PC IgE (206)–sensitized wild-type cells incubated with antigen for the indicated periods also attracted Fc∈;RIα−/− cells. (C) Migration was induced by adenosine and LTB4, but not by sphingosine 1–phosphate (S1P) or histamine. (D) Pertussis toxin (PTX), adenosine deaminase (ADA), and adenosine A3 receptor inhibitor MRS 1523 inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen or adenosine. (E) 5-Lipoxygenase inhibitor (N-oleoyl dopamine [ODA]), BLT1 receptor inhibitor (U-75302), and BLT2 inhibitor (LY2552833) inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. U-75302 and LY2552833 at 1 μM each were used in a combination (U+LY) as well. (F) Neutralizing antibodies to several chemokines inhibit the migration of CFSE-labeled wild-type BMMCs from upper wells to lower wells containing IgE-sensitized wild-type cells and antigen. All the antibodies at the higher concentrations each were used in a combination (all) as well. Mean values ± SD are shown out of 2 independent experiments.