Figure 6. OEA induces visceral pain and decreases short-term food intake in mice via TRPV1.

A, wild-type mice (C57Bl6) were injected with 25 mg kg⁻¹ OEA (filled bar, n = 5) or 25 mg kg⁻¹ OEA plus 20 mg kg⁻¹ capsazepine (striped bar, n = 5). The time spent recumbent (either lying on stomach or sitting hunched and immobile) was recorded for the first 10 min after injection. TRPV1-null mice were injected with 25 mg kg⁻¹ OEA (n = 6) and spent no time recumbent. *P < 5.0 × 10⁻⁵**P < 5.0 × 10⁻⁸ compared with wild-type injected with OEA. B, wild-type and TRPV1-null mice were fasted for 24 h prior to experiment. Mice were injected with either 12.5 mg kg⁻¹ OEA or control solution (100% DMSO). Mice were immediately re-introduced to food and food intake was monitored after 30 min, food intake was measured as grams of food consumed per 100 g body weight. Bars show difference in each mouse injected with OEA from the mean food intake of the control group, n = 4–5 for each group. **P < 5.0 × 10⁻⁷ compared with wild-type mice.