Figure 3. Effect of osmotic stress on the abundance of HSP27 and HSP70.

MDCK and PI cells were incubated for 24 h with the indicated solutes, or maintained in isotonic medium. Subsequently, the expression of HSP27 and HSP70 was determined by Western blot analysis. To demonstrate equal protein loading, the blots were also probed with β-actin antiserum. Data are means ± s.e.m. for 3–4 separate experiments; *P < 0.05 versus respective isotonic control (iso).