Table 1.

Primers used for PCR amplification of cDNA

Product	Accession no.	Sequence	Position	TA (°C)	Cycles	Product length (bp)	Restriction enzyme	Restriction products
Bcl-2	NM_016993	F: 5′-CCGGGACGCGAAGTGCTATTGGTAC-3′	187–211	55	39	950	BamHI	602, 348
		R:5′-AGCTGATTTGACCATTTGCCTGAA-3′	1113–1136				Bg1I	686,264
							BstXI	572, 438
							DraI	865, 85
Bax	AF235993	F:5′-GCACCCCTTTCCTCCTCTCTCCACCAG-3′	462–488	55	39	654	BamHI	445, 209
		R:5′-TGCCTTTCCCCGTTCCCCATTCATC-3′	1091–1115				PstI	431, 223
							BstXI	521, 133
AIF	AF375656	F:5′-CCGGCTTCCAGGCAACTTGTTCC-3′	93–115	58.1	33	359	KpnI	290, 69
		R:5′-CCCGGATGGATCTAGCTGCTGCA-3′	429–451				PstI	259, 100
Apaf-1	AF218388	F:5′-CGGCCCTGCGCATCTGATTCAT-3′	1623–1644	57.8	36	288	AluI	193, 95
		R:5′-GGGCGAACGACTAAGCGGGACAG-3′	1888 –1910				PstI	198, 90
Caspase-3	NM_012922	F:5′-TGGCCCTGAAATACGAAGTC-3′	262–281	53.3	36	339	RsaI	260, 79
		R:5′-CGGCCTCCACTGGTATCT-3′	583–600				SpeI	183, 156
							AluI	266, 73
Caspase-9	NM_031632	F:5′-GGCCGGTGGACATTGGTTCTGG-3′	543–564	55	36	222	BamHI	130, 92
		R:5′-CCATGAAGCGCAGCCAGCAGAA-3′	743–764				HindIII	186, 36

T_A, annealing temperature; Accession no., GenBank accession number; F, forward primer; R, reverse primer.