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. 2005 Mar 17;565(Pt 1):279–294. doi: 10.1113/jphysiol.2005.082776

Figure 8. Effect of AMPA receptor blockade after pretreatment with 7-NI.

Figure 8

CNQX (0.5 mm) was topically applied in five out of eight animals treated with 7-NI and responses evoked by climbing fibre stimulation at 10 Hz were compared. A, shows an example of Ptiss,O2 and corresponding CBF responses from one animal under control conditions (black trace), after application of 7-NI (dark green), in the presence of 7-NI + CNQX (red) and after washout of CNQX (bright green). Grey bars denote the stimulation period. B, shows the averaged responses before (CTR) and after application of 7-NI (7-NI) and illustrates the temporary effect of CNQX (CNQX and Wash out). As 7-NI is an irreversible nNOS inhibitor, it cannot be washed out. Compared to 7-NI values, the negative Ptiss,O2 response during application of CNQX was reduced by ∼94%. The already diminished positive Ptiss,O2 and CBF responses were both further reduced by ∼91%. However, the decrease in the positive Ptiss,O2 response did not reach significance. CNQX application attenuated the ΣLFP by 85% (compared to 7-NI; P < 0.05; data not shown). All CNQX effects were reversible with no significant differences to 7-NI. All values are given as mean ± s.e.m.*P < 0.05 compared to 7-NI.