Table 1.
Mean values (± s.e.m.) of the Boltzmann fits (V0.5 and k) to activation and fast inactivation curves obtained for A1152D mutant and WT at 21°C and 11°C
| Activation | Fast inactivation | ||||||
|---|---|---|---|---|---|---|---|
| Erev(mV) | V0.5(mV) | k(mV (e-fold change)−1) | V0.5(mV) | k(mV (e-fold change)−1) | IssIIpeak(%) | ||
| Nernst | 80.5 | ||||||
| 21°C | A1152D | 81.1 ± 0.9 (25) | −18.6 ± 0.4 (25) | 7.2 ± 0.1 (25) | −65.7 ± 0.5 (8) | 6.9 ± 0.2 (8) | 0.96 ± 0.14 (5) |
| WT | 81.3 ± 0.8 (17) | −19.6 ± 0.7 (17) | 7.3 ± 0.1 (17) | −71.3 ± 0.4 (10) | 6.3 ± 0.3 (10) | 0.78 ± 0.07 (6) | |
| Nernst | 77.7 | ||||||
| 11°C | A1152D | 76.7 ± 0.4 (12) | −13.5 ± 0.4 (12) | 8.2 ± 0.1 (12) | −64.8 ± 0.3 (10) | 7.7 ± 0.4 (10) | 4.42 ± 0.62 (7) |
| WT | 76.9 ± 0.6 (15) | −15.1 ± 0.4 (15) | 8.2 ± 0.1 (15) | −73.7 ± 0.3 (11) | 6.4 ± 0.3 (11) | 1.55 ± 0.35 (9) | |
The last column indicates the fraction of sustained current (Iss/Ipeak× 100) measured at the end of a 200 ms pulse to −10 mV.