Figure 1. Cytolysis of wild-type mouse myotubes in lactate dehydrogenase (LDH) release cytotoxicity assays.

A, muscle cytolysis in the absence (Muscle only) or presence (Muscle + Neutrophils) of wild-type neutrophils. Exogenous superoxide dismutase (SOD) was added to some preparations. All treatments used 5000 neutrophils mm⁻². Data from cultures that were not subjected to mechanical loading are shown by black columns. Data from cultures subjected to mechanical loading are shown by grey columns. B, identical treatments as shown in A, but neutrophils from myeloperoxidase (MPO) −/− mice were used in the assays. * Significantly different from corresponding non-loaded treatment. # Significantly different from muscle cells without neutrophils treated under otherwise identical conditions. P < 0.05. n = 6 replicates per treatment. Bars represent s.e.m.