. 2005 Apr 7;565(Pt 3):717–730. doi: 10.1113/jphysiol.2005.084988

Table 1.

Effect of mutating residues in the intracellular loops, N- and C-termini on kNBC1 function

Plasmid	pH_i minimum	Total buffer capacity (mm pH⁻¹)	Na⁺-dependent base flux (mm min⁻¹)	Na⁺-dependent base flux (% of wt)
wt-kNBC1	6.68 ± 0.19	38.1 ± 2.08	22.5 ± 1.84	100 ± 8.21
Vector	6.70 ± 0.06	39.4 ± 5.69	0 ± 0.05^*	0^*
D416N	6.65 ± 0.02	35.5 ± 2.21	19.5 ± 1.38	86.7 ± 6.15
D419N	6.65 ± 0.03	34.5 ± 2.66	24.4 ± 1.69	108 ± 7.52
K667R	6.69 ± 0.19	39.0 ± 8.62	23.8 ± 3.61	106 ± 16.0
K667E	6.65 ± 0.28	34.6 ± 2.49	4.16 ± 0.32^*	18.5 ± 1.40^*
K667N	6.67 ± 0.02	34.1 ± 2.53	8.27 ± 1.04^*	36.8 ± 4.64^*
K667Q	6.67 ± 0.07	35.1 ± 5.95	5.79 ± 0.48^*	25.8 ± 2.14^*
K668N	6.71 ± 0.02	36.1 ± 1.82	6.82 ± 0.62^*	30.4 ± 2.78^*
⁶⁶⁷KK-⁶⁶⁷NN	6.71 ± 0.03	36.7 ± 2.47	2.27 ± 0.19^*	10.1 ± 0.86^*
⁶⁶⁷KK-⁶⁶⁷QQ	6.70 ± 0.02	34.1 ± 1.53	0 ± 0.04^*	0^*
K670Q	6.69 ± 0.03	38.7 ± 3.10	16.0 ± 2.30^*	71.1 ± 10.0^*
T671N	6.54 ± 0.02	35.8 ± 1.72	3.10 ± 0.19^*	13.8 ± 0.87^*
T677G	6.74 ± 0.04	40.4 ± 2.98	12.9 ± 1.52^*	57.5 ± 6.76^*
R680Q	6.65 ± 0.02	36.8 ± 1.34	21.0 ± 1.97	93.0 ± 8.74
R680E	6.62 ± 0.02	34.3 ± 2.56	5.35 ± 0.30^*	23.8 ± 1.33^*
K681N	6.72 ± 0.05	35.5 ± 0.04	9.96 ± 0.64^*	44.3 ± 2.82^*
⁶⁸⁰RK-⁶⁸⁰NN	6.69 ± 0.02	35.1 ± 1.44	4.94 ± 0.48^*	22.0 ± 2.12^*
D754E	6.51 ± 0.02	35.4 ± 1.99	5.26 ± 0.43^*	23.3 ± 1.90^*
D754N	6.69 ± 0.04	37.8 ± 5.92	1.94 ± 0.16^*	8.64 ± 0.74^*
D754R	6.66 ± 0.07	35.0 ± 7.37	0 ± 0.27^*	0^*
⁷⁶⁴RK-⁷⁶⁴NN	6.67 ± 0.14	38.4 ± 5.66	14.2 ± 1.47^*	63.2 ± 6.53^*
⁷⁶⁷HK-⁷⁶⁷NN	6.68 ± 0.06	39.4 ± 5.73	21.3 ± 3.87	94.7 ± 17.2
⁷⁷⁰KK-⁷⁷⁰NN	6.67 ± 0.26	37.8 ± 2.19	9.29 ± 0.73^*	41.3 ± 3.25^*
⁷⁶⁷HKLKK- ⁷⁶⁷NNLNN	6.68 ± 0.03	38.1 ± 2.82	8.97 ± 0.90^*	39.9 ± 4.00^*
H776D	6.70 ± 0.03	35.1 ± 2.90	18.0 ± 1.10^*	80.0 ± 4.91^*
H776N	6.75 ± 0.02	40.5 ± 3.76	12.2 ± 0.86^*	54.1 ± 5.83^*
H776S	6.75 ± 0.03	38.3 ± 3.14	9.56 ± 0.75^*	42.5 ± 3.33^*
H776R	6.70 ± 0.03	38.1 ± 2.82	8.97 ± 0.90^*	39.9 ± 4.00^*
D778N	6.64 ± 0.24	35.2 ± 2.41	5.77 ± 0.24^*	25.6 ± 1.07^*
⁸⁹⁰KH-⁸⁹⁰NN	6.72 ± 0.03	38.4 ± 3.07	25.0 ± 1.48	111 ± 6.60
⁸⁹⁹RH-⁸⁹⁹NN	6.75 ± 0.04	38.6 ± 4.32	25.1 ± 1.79	112 ± 7.97
R904E	6.59 ± 0.02	35.3 ± 4.23	5.45 ± 0.27^*	24.2 ± 1.47^*
R905E	6.60 ± 0.02	34.2 ± 0.40	10.8 ± 1.02^*	48.1 ± 4.55^*
⁹⁰⁴RR-⁹⁰⁴QQ	6.74 ± 0.02	37.4 ± 2.44	19.1 ± 2.85^*	85.0 ± 12.7
R943N	6.68 ± 0.04	37.2 ± 3.63	8.74 ± 1.01^*	38.9 ± 4.60^*
R943E	6.56 ± 0.07	37.0 ± 9.70	12.9 ± 1.47^*	57.3 ± 6.53^*

P < 0.05 versus wt-kNBC1.