. 2005 Apr 7;565(Pt 3):717–730. doi: 10.1113/jphysiol.2005.084988

Table 3.

Effect of mutating residues in the extracellular loops on kNBC1 function

Plasmid	pH_i minimum	Total buffer capacity (mm pH⁻¹)	Na⁺-dependent base flux (mm min⁻¹)	Na⁺-dependent base flux (% of wt)
E459Q	6.67 ± 0.04	34.5 ± 2.94	2.02 ± 0.12^*	8.99 ± 0.55^*
R538N	6.73 ± 0.04	37.8 ± 4.19	11.5 ± 0.92^*	51.1 ± 4.19^*
R538Q	6.66 ± 0.02	34.1 ± 1.75	4.79 ± 0.36^*	21.3 ± 1.61^*
E541Q	6.78 ± 0.04	38.2 ± 2.65	19.3 ± 2.26	85.7 ± 10.10
E542Q	6.63 ± 0.02	38.8 ± 1.45	7.55 ± 0.08^*	33.6 ± 0.36^*
D555N	6.49 ± 0.04	38.8 ± 3.61	12.8 ± 1.04^*	56.9 ± 4.62^*
A556T	6.61 ± 0.04	34.6 ± 3.35	1.68 ± 0.14^*	7.48 ± 0.64^*
K558Q	6.54 ± 0.03	35.4 ± 2.81	12.0 ± 0.96^*	53.4 ± 4.27^*
K559Q	6.70 ± 0.03	39.8 ± 3.25	0.97 ± 0.05^*	4.30 ± 0.22^*
⁵⁵⁸KK-⁵⁵⁸QQ	6.77 ± 0.03	41.0 ± 2.98	3.28 ± 0.24^*	14.6 ± 1.06^*
Y567H	6.61 ± 0.05	35.6 ± 1.81	20.6 ± 1.88	91.8 ± 8.34
D647R	6.62 ± 0.02	34.7 ± 0.42	2.43 ± 0.21^*	10.8 ± 0.95^*
D705N	6.68 ± 0.19	35.6 ± 1.58	8.82 ± 0.99^*	39.2 ± 4.40^*
R722Q	6.65 ± 0.04	38.2 ± 1.72	10.8 ± 1.30^*	48.1 ± 5.78^*
E731N	6.77 ± 0.02	42.0 ± 2.28	16.4 ± 1.48^*	72.9 ± 6.56^*
D809N	6.66 ± 0.02	35.2 ± 1.82	11.4 ± 0.75^*	50.7 ± 3.30^*
K812T	6.76 ± 0.03	36.4 ± 3.48	20.4 ± 3.11	90.6 ± 13.9
⁸¹⁴ETE-⁸¹⁴MSK	6.68 ± 0.19	37.0 ± 3.07	7.18 ± 0.20^*	31.9 ± 1.86^*
G828E	6.65 ± 0.07	36.0 ± 3.45	25.8 ± 1.84	114.7 ± 8.18
E831Q	6.68 ± 0.02	35.0 ± 2.23	7.22 ± 0.82^*	32.1 ± 3.63^*
⁸³¹EQR-⁸³¹QQQ	6.63 ± 0.03	35.6 ± 1.85	0.71 ± 0.04^*	3.17 ± 0.18^*
K924Q	6.78 ± 0.02	41.6 ± 2.28	8.11 ± 0.44^*	36.1 ± 1.96^*

P < 0.05 versus wt-kNBC1.