Figure 4. Quantitative real-time PCR to test our microarray results for consistency.

A and B, amplification curves for genes ARP and Serpinh1 from AM total RNA exposed to normoxia (dashed lines) or 10% O₂ (continuous lines) for 24 h. C, relative quantification of mRNA expression by PCR for five genes was calculated by the 2^−ΔΔC_T method using m-Gus as an internal control. Error bars represent standard deviations obtained from triplicate amplifications. These results are shown compared to gene transcription obtained in our array experiments relative to m-Gus. The dashed line marks the baseline for transcription set by the internal control mRNA.