Table 1.
Parameters used to evaluate the quality of the microarray hybridization signals
Scale factor | M/3′ | Present calls | Concordant calls | Reliability index | |
---|---|---|---|---|---|
CB normoxia | 0.73 | 3.87 | 47.40 | 84.5 | 0.98 |
CB hypoxia | 0.77 | 3.17 | 49.57 | 85 | 0.99 |
AM normoxia | 0.55 | 1.80 | 51.80 | 83 | 0.98 |
AM hypoxia | 0.78 | 1.76 | 51.47 | 84.3 | 0.98 |
The M/3′ value was obtained from the m-GAPDH control probe sets. The reliability index is the Cronbach's α coefficient estimated from multiple regression analysis of the triplicate hybridization values.