Skip to main content
. 2005 Apr 21;566(Pt 2):301–308. doi: 10.1113/jphysiol.2004.080200

Figure 4. Patch-clamp analysis of stably transfected TRPM6/7 in HEK293 cells.

Figure 4

TRPM6 and TRPM7 were each stably transfected into HEK293 cells by using an ecdysone inducible gene expression system (Invitrogen) that allows the tightly controlled expression of transfected constructs by induction with Ponasterone A (5 μm). Whole-cell patch clamp recordings were performed 18–24 h after induction. Without induction, small endogenous TRPM7 currents are observed (green curve). Induction of stably transfected TRPM6 does not result in a significant increase in currents (red curve), whereas induction of a stably transfected TRPM7 results in the development of large currents with outward rectification at positive potentials (blue curve) as described before (Runnels et al. 2001; Nadler et al. 2001). Additional transient expression of TRPM6 (Fugene6, Roche) in the Ponasterone-induced TRPM7 cell line leads to a significant amplification of TRPM7-like currents (black curve).