Figure 2. Representative coronal images of brain sections illustrating relative expression patterns of mRNA encoding deiodinase type 2 (D2) following a rostral–caudal gradient from the forebrain to the brainstem (note: not all areas illustrated).

D2 mRNA (A–D) expression was detected in the lining of the lateral ventricles (LV) and shell of the nucleus accumbens (ACBsh), cerebral cortex, cingulate cortex (Cg) and hippocampal formation, with low levels in the central nucleus of amygdala (CeA). D2 mRNA was expressed throughout the brainstem and cerebellum with particularly high levels being detected in the cochlear nuclei (CN), cerebellar flocculus (FL), and vestibular nucleus (VN). Deiodinase type 3 mRNA (D3, F and G) was found specifically in the capsule of the amygdalohippocampal nucleus (AHiPM) and posteromedial cortical amygdaloid nucleus (PMCo) in the forebrain but was more diffusely expressed in the brainstem (BS) and cerebellum (CB). As a negative control, sense probes for the deiodinase subtypes were made. Sections hybridized with sense probes for D2 (E) and D3 mRNA (H); no signal was detected.