Figure 7. Mechanism of inhibition of NO release by 4 Hz BS.
A, effects of extracellular Ca2+ (2 mm and 8 mm) on NO release in response to 60 BSs repetitively applied at 1 Hz (open bars) and 4 Hz (filled bars). B, effects on the BS-induced NO release of specific antagonists of type I mGluR (CPCCOEt, 100 μm). C and D, peak values of NO release in response to 60 BSs applied at 1 Hz and 4 Hz in the absence and presence of the CB1 receptor antagonist AM281 (2 μm) or CB1 receptor agonist WIN55,212-1 (5 μm). Mean ± s.e.m. (n = 5).