Fig. 5.

Effects of NOS inhibitor on the levels of NMDA stimulated increases in NO-IF. (A) A contrast reversed image of a DAF loaded retinal slice that was pre-incubated with the NOS inhibitor 100 μM LNMMA. In the absence of stimulation there was some faint NO-IF in some somata in the INL and GCL. (B) Even 400 s after stimulation with 250 lM NMDA there was still no increase in NO-IF which indicates that NMDA stimulated increases in NO-IF require the presence of enzymatically active NOS. Notice the decrease in endogenous NO-IF after 400 s of illumination. Scale bar, 20 lm.