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. 2006 Jun;12(6):1092–1103. doi: 10.1261/rna.2230106

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FIGURE 3. — In vivo analysis of M. jannaschii and A. fulgidus box C/D sRNP core proteins reveal organism-specific protein:protein interactions. M. jannaschii and A. fulgidus core protein interactions were assessed in vivo using the yeast two-hybrid system. Yeast carrying a Gal4:HIS3 reporter gene were transformed sequentially with a bait vector (indicated on top) and a prey vector (indicated on bottom), and the resulting strains were tested for growth. (A) M. jannaschii (Mj) and A. fulgidus (Af) L7 core protein interactions with L7, Nop56/58, and fibrillarin. (B) M. jannaschii (Mj) core protein interactions between Nop56/58 and fibrillarin. (C) A. fulgidus (Af) core protein interactions between Nop56/58 and fibrillarin. (D) M. jannaschii (Mj) Nop56/58 coiled-coil mutant core protein interactions between mutant Nop56/58 and fibrillarin proteins.