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CUG-BP binds to AREs and to sites outside those regions. (A) UV cross-linking was performed using c-fos and TNFα RNA substrates and variant RNAs that lacked the ARE. The CUG-BP band and molecular weight markers are indicated. (B) Increasing picomolar amounts of Region A of the c-fos RNA (see Fig. 1) or a mutant version were used as competitor RNAs in UV cross-linking reactions. The position of the CUG-BP band is indicated.
