FIGURE 6.

(A) CUG-BP and PARN coimmunoprecipitate. CUG-BP was immunoprecipitated from HeLa extracts with anti-CUG-BP antibodies and the immunoprecipitate was separated on a 10% gel containing SDS and tested for the presence of PARN by Western blotting. A mock immunoprecipitation was performed with normal serum as a control. (B) CUG-BP and PARN interact directly. Recombinant 6HisPARN was incubated in the presence of RNAse A with glutathione Sepharose beads (PARN alone) or with similar beads that had been prebound with GST-CUG-BP (GST-CUG-BP + PARN) or GST (GST + PARN). After extensive washing, the bound proteins were separated by SDS-PAGE and PARN was detected by Western blot.