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Duplexes greater than 23 bp in length induce toxicity in HeLa S3 cells. Bar graph showing the level of DBI silencing (A) and viability of HeLa S3 cells (B) transfected with DBI 32 dsRNA of varying duplex lengths (17–31 bp) at concentrations ranging from 0.1 to 100 nM. Experiments were performed in triplicate. Error bars represent standard deviations from the mean.
