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Nonspecific dsRNA-mediated cytotoxicity induced by long siRNA is unique to specific cell types. The effect of six different long (27 bp) dsRNAs and siRNAs (19 bp) targeting DBI on cell viability in MCF7 (A), DU 145 (B), HeLa S3 (C), HeLa (D), and HEK293 (E) cells at 100 nM concentrations. Experiments were all performed in triplicate. Error bars represent standard deviations from the mean. RF represents RISC-free control.
