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E2 inhibition of uterine sGC mRNA levels occurs independently of new protein synthesis. Ovariectomized rats were pretreated with puromycin (30 min) and subsequently administered E2 (40 μg/kg). Animals were killed 3 h after the administration of E2, and sGC α1 and β1 mRNA levels were determined by real-time Q-PCR. Mean values ± SEM (n = 3) were normalized to 36b4 and graphed as percent of control.
